A 4-pronged approach to foster healthy aging in older adults.

Monitoring patients\u27 health, mobility, mentation, and ability to maintain social connections can help you promote healthy aging for your older patients

Movement and Countermovement Dynamics Between the Religious Right and LGB Community Arising from Colorado’s Amendment 2

This sample of the case study of Equality Colorado will demonstrate how counter movements and litigation may limit and change how an organization surrounding a social movement acts. Colorado for Family Values helped pass Colorado’s Amendment 2 in 1992, which limited any present and future anti-discrimination legislation that would protect sexuality as a class. This ballot initiative passed by 53% of Colorado voters, and other states like Idaho and Oregon attempted to replicate this type of initiative. Amendment 2 challenged the LGB community and compelled the movement to collectively respond to the religious right with coalitions, pooled resources, and litigation. Equality Colorado, established in 1992, will exemplify how a social movement could respond to prejudicial legislation. One of Equality Colorado’s primary tactics was to reframe religion as inclusive of gay rights. It did not cede religion entirely to its opponents and attempted to delegitimize them by labeling them “radical right” as opposed to the more popular term “religious right” or “Christian Conservatives”. Additionally, Equality Colorado tried to compensate for the downsides of litigation by “translating” the legal terms to the general public and connecting litigators with the broader movement

Biogenesis of a Bacterial Organelle: The Carboxysome Assembly Pathway

SummaryThe carboxysome is a protein-based organelle for carbon fixation in cyanobacteria, keystone organisms in the global carbon cycle. It is composed of thousands of subunits including hexameric and pentameric proteins that form a shell to encapsulate the enzymes ribulose 1,5-bisphosphate carboxylase/oxygenase and carbonic anhydrase. Here, we describe the stages of carboxysome assembly and the requisite gene products necessary for progression through each. Our results demonstrate that, unlike membrane-bound organelles of eukaryotes, in carboxysomes the interior of the compartment forms first, at a distinct site within the cell. Subsequently, shell proteins encapsulate this procarboxysome, inducing budding and distribution of functional organelles within the cell. We propose that the principles of carboxysome assembly that we have uncovered extend to diverse bacterial microcompartments

Early Holocene Occupation at the West Lost River Site, Klamath County, Oregon

Excavations at the West Lost River Site (35KL972) provide new insights on early Holocene occupation of southwestern Oregon. The article focuses on the artifacts and specimens recovered from the site

Enhanced mtDNA repair and cellular survival following oxidative stress by targeting the hOGG repair enzyme to mitochondria.

Oxidative damage to mtDNA has been implicated as a causative factor in many disease processes and in aging. We have recently discovered that different cell types vary in their capacity to repair this damage, and this variability correlates with their ability to withstand oxidative stress. To explore strategies to enhance repair of oxidative lesions in mtDNA, we have constructed a vector containing a mitochondrial transport sequence upstream of the sequence for human 8-oxoguanine glycosylase. This enzyme is the glycosylase/AP lyase that participates in repair of purine lesions, such as 8-oxoguanine. Western blot analysis confirmed this recombinant protein was targeted to mitochondria. Enzyme activity assays showed that mitochondrial extracts from cells transfected with the construct had increased enzyme activity compared to cells transfected with vector only, while nuclear enzyme activity was not changed. Repair assays showed that there was enhanced repair of oxidative lesions in mtDNA. Additional studies revealed that this augmented repair led to enhanced cellular viability as determined by reduction of tetrazolium compound to formazan, Trypan blue dye exclusion, and clonogenic assays. Therefore, targeting of DNA repair enzymes to mitochondria may be a viable approach for the protection of cells against some of the deleterious effects of oxidative stress

Effects of Occupational Ultrasonic Noise Exposure on Hearing of Dental Hygienists: A Pilot Study

Purpose. The purpose of this case-control study was to determine whether long-term ultrasonic noise exposure via the dental office environment is related to dental hygienists\u27 hearing status. Methods. Registered dental hygienists (N - 698) who live in the Hampon Roads areas of Virginia were mailed a Dental Hygiene Word History Questionnaire to determine who would meet the inclusion criteria and would be willing to participate in the study. Consenting subjects were categorized into one of two groups, according to ultrasonic sealer usage rate, and matched on age. Persons with known hearing loss due to infection, disease, or congenital defect were excluded from the study. The final sample consisted of 20 dental hygienists with a high ultrasonic usage rate and a matched group of 20 dental hygienists who had a low ultrasonic usage rate. Once the groups were formed, a certified audiologist tested subjects\u27 hearing in each ear via the pure-tone audiometer. Audiometric data were analyzed using the analysis of variance for repeated measures procedure to determine if degree of ultrasonic sealer noise exposure in the dental office environment was significantly related to hearing status in these dental hygienists. Results. Results revealed that the right and left ears were not statistically different in the hearing threshold levels, regardless of group status. However, there was a significant difference in the high ultrasonic usage group and the low ultrasonic usage group at the 3000 Hz. No differences were found at the frequencies of 500, 1000, 2000, 4000, 6000, and 8000 Hz. Conclusions. Based on these outcomes, the ultrasonic sealer is not considered to have a negative effect on the hearing of dental hygienists at 500,1000, 2000, 4000, 6000, and 8000 Hz, but may be related to hearing loss at 3000 Hz. Ultrasonic noise may in fact be affecting dental hygienists\u27 hearing at 3000 Hz, but loss of hearing observed at the higher frequencies may be attributed to other unidentified factors present in both groups

Altering DNA Base Excision Repair: Use of Nuclear and Mitochondrial-Targeted N-Methylpurine DNA Glycosylase to Sensitize Astroglia to Chemotherapeutic Agents

Primary astrocyte cultures were used to investigate the modulation of DNA repair as a tool for sensitizing astrocytes to genotoxic agents. Base excision repair (BER) is the principal mechanism by which mammalian cells repair alkylation damage to DNA and involves the processing of relatively nontoxic DNA adducts through a series of cytotoxic intermediates during the course of restoring normal DNA integrity. An adenoviral expression system was employed to target high levels of the BER pathway initiator, N-methylpurine glycosylase (MPG), to either the mitochondria or nucleus of primary astrocytes to test the hypothesis that an alteration in BER results in increased alkylation sensitivity. Increasing MPG activity significantly increased BER kinetics in both the mitochondria and nuclei. Although modulating MPG activity in mitochondria appeared to have little effect on alkylation sensitivity, increased nuclear MPG activity resulted in cell death in astrocyte cultures treated with methylnitrosourea (MNU). Caspase-3 cleavage was not detected, thus indicating that these alkylation sensitive astrocytes do not undergo a typical programmed cell death in response to MNU. Astrocytes were found to express relatively high levels of antiapoptotic Bcl-2 and Bcl-XL and very low levels of proapoptotic Bad and Bid suggesting that the mitochondrial pathway of apoptosis may be blocked making astrocytes less vulnerable to proapoptotic stimuli compared with other cell types. Consequently, this unique characteristic of astrocytes may be responsible, in part, for resistance of astrocytomas to chemotherapeutic agents